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KMID : 0382420000260040088
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Korean Journal of Environmental Health Society
2000 Volume.26 No. 4 p.88 ~ p.96
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Changes on the Methylmercury-induced Cytotoxicity by Control of Cell Membrane Transport System
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Youm Jung-Ho
Kim Nam-Song
Koh Dai-Ha
Kim Jun-Youn
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Abstract
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The aim of the current study was to evaluate the transport system in EMT-6 cell for the uptake of the methylmercury(MeHg). Several inhibitors were used to test which potential transport system might be involved in MeHg uptake. Probenecid was used to test the organic transport system, valinomycin for testing the effect of the membrane potential, cytochalasin B for testing the facilitated diffusive D-glucose transport system and colchicine for testing the microtubule system. Ouabain for evaluating active transport system, 4´,4-diisothiocyano-2´,2-stilbenedisulfonic acid(DIDS) the Cl^- ion transport system and verapamil for the Ca^2+ transport system. Significantly, MeHg decreased the synthesis of nitric oxcide(NO) and intracellular ATP in EMT-6 cells. In the condition of ouabain containing with MeHg, intracellular ATP level and NO production was not decreased. But other inhibitors containing with MeHg decreased the production of NO and intracelluar ATP. In the treatment of inhibitors, ouabain showed protective effect against cytotoxicity of MeHg but other inhibitors not showed protective effects. The protective effects of ouabain against the cytotoxicity of McHg depended on the concentration of added ouabain to the culture medium for EMT-6 cells. These result showed that the uptake of MeHg might be involved in the active transport system. Active transports system seems to share similarities with the transport systems for the uptake of MeHg when using MeHg and MeHg-glutathione complex.
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